Table 1(comtinued DNA fragment used in NMR Summary of direct protein-DNA interactions Nucleic acid side-chains TFIIIA CSFADCGAAYNKNWKLQAH TIGGATGGGAGACC Gual4. Gua13 and Thy 12 strand B Gua9, Ade 10 and GualI strand a: Thy 12 GCEKGFTSLHHLTR上 Ade 10 and GualI strand a INRFH Three Cyu strand B Gua3 and Gua4 strand a: Thy s strand B Arg2 Gua 3 strand a Arg96 ZINC FINGER C2H2 ICEVCTRAFARQEHLKRH' CCATCTCCAACTTAT Adeb and Gua7 strand a: Thy& strand GA "CPICYAVIRQSRNLRRHLELRH GCCGAGAGTAG Cyto strand B: Ade7 strand a ys strand B: Ades strand a leg strand 8 GCACAAAC Cyu and Ades strand a: Guas strand B GYQWKMLTEAR Ades and Ade6 srand Guas strandN. Jamin, F. Toma / Progress in Nuclear Magnetic Resonance Spectroscopy 38 (2001) 83±114 95 Table 1 (continued) Protein name Prosite pattern Pattern/protein sequencea DNAb fragment used in NMR study Summary of direct protein±DNA interactions Ref. Amino acid side-chains Nucleic acid side-chains TFIIIA ZINC_FINGER_C2H2 15CSFADCGAAYNKNWKLQAH TTGGATGGGAGACC Finger 1: Lys26 Gua14, Gua13 and Thy12 strand b [64] LSKH G Finger 1: Trp28 Gua9, Ade10 and Gua11 strand a; Thy12 strand b 45CKEEGCEKGFTSLHHLTRH SLTH Finger 1: Lys29 Ade10 and Gua11 strand a 75CDSDGCDLRFTTKANMKKH Finger 2: His58 Thy10 strand b FNRFH Finger 2: His59 Gua8 and Gua9 strand a Finger 2: Arg62 Gua7 strand a Finger 3: Thr86 Thy6 strand a Finger 3: Ala88 Cyt7 strand b Finger 3: Asn89 Ade5 strand a Finger 3: Arg92 Gua3 and Gua4 strand a; Thy5 strand b Finger 3: Arg96 Gua 3 strand a ADRI ZINC_FINGER_C2H2 106CEVCTRAFARQEHLKRHY CCATCTCCAACTTAT d [65] RSH AAGTTGGAGATCC GATA1 GATA_ZN_FINGER 7CSNCQTSTTTLWRRSPMGD GTTGCAGATAAACAT Thr16 Ade9 and Thy8 strand b [46] PVCNAC T Leu17 Ade6 and Gua7 strand a; Thy8 strand b Asn29 Ade8 strand a; Ade9 strand b Leu33 Ade9 and Thy10 strand b Leu37 Thy10 and Thy11 strand b Lys57 Thy9 strand a GAGA ZINC_FINGER_C2H2 36CPICYAVIRQSRNLRRHLELRH GCCGAGAGTAG Arg14 Cyt6 strand b; Ade7 strand a [66] Lys16 Cyt4 and Thy5 strand b; Ade5 strand a Ser26 Thy10 strand b Arg27 Gua8 strand a Ser30 Cyt8 and Ade9 strand b Arg47 Gua6 strand a; Thy7 strand b Asn48 Ade5 strand a Arg51 Gua4 strand a SRY human ± 1VQDRVKRPMNAFIVWSRDQ GCACAAAC Asn10 Cyt4 and Ade5 strand a; Gua5 strand b [70] RRKMALENPRMRNSEISKQL Phe12 Thy5 and Thy6 strand b GYQWKMLTEAEKWPFFQEAQ Ile13 Ade5 and Ade6 strand a KLQAMHREKYPNYKYRP Ser33 Gua8 strand b