Sequences upstream of the J regions of the kappa locus Recombination signal sequences- 9-mer 7-mer ÷609r. CACTOTG conserved sequences (23bp) in regions just upstream or (23bp) Tyr Thr Phe Gly TGCTCAGTTTTTGTATGGGGGTTGAGTGAAGGGACACCAGTGTGTGTACACGTTCGGA downstream of gene segments. (21 bp) lle Thr Phe Ser CCTAGGGAGGGTTTTGTGGAGGTAAAGTTAAAATAAATCACTGTAAATCACATTCAGT Consist of a conserved AGGCAGGGAAGCGGGGCCCAGTGATATGAACATICACGTICCGC (24bp) heptamer and nonamer with a 12 or CAGOCAGAGACGGOCATOTCATAGTCCICACTOTOBCTCACOTTCOO (23bp) 23 bp spacer. (b)K-chain DNA n=-85 LVx1 LVx2 LVn □□…☑ 1-1-31 23 2.5 kb kb Kuby Figure 5-3 Recombination signal sequences - conserved sequences in regions just upstream or downstream of gene segments. Consist of a conserved heptamer and nonamer with a 12 or 23 bp spacer. Kuby Figure 5-3