南 Hpal 6000 Hindlll Hindlll Hpal EcoRV ori 1000 Bglll e 5000 Xhol 一Hindlll Clal- Smal- kan pcos1EMBL (6.1kb) EcoRI 园 Hindlll一 Hindlll 2000 4000 BamHI EcoRl tetr BstEll 300032