Shotgun sequencing a bac or a genome 200 kb(NIH 3 Gb(Celera) Sonicate. Subclone Subclones Sequence, Assemble What would cause problems W Shotgun Contigs assembly?Shotgun Sequencing a BAC or a Genome 200 kb (NIH) 3 Gb (Celera) Sequence, Assemble Sonicate, Subclone Subclones Shotgun Contigs What would cause problems with assembly?