ortional to the number of genes contained within that genome. In other words, a ome that is 10 times as big will contains approximately 10 times as many protein coding genes 1)True 2)False 13. HeLa cells, derived from a human cervical carcinoma, are able to propagate indefinitely in culture and are therefore known as a(n) 1) tissue culture 2)tu 3)transgenic cell line 4)immortalized cell line 14. E. coli cells are smaller than yeast cells 2)False 15. Which of the following domains is not a dna bind ing domain 1) Proline- rich domains 2)Helix-turn helix domains 3)Zinc finger domains 16. The aminoacyl-tRNa synthetases distinguish between about 40 different shaped tRNA molecules in the cells 1)True 2) False PART III: SHORT QUESTIONS (8 points each) 1. How do bacterial replication start and accomplished Remember to include the proteins/enzymes and important dna sequence involved in this process 2. Design experiments to clone a yeast gene and express this gene in yeast 3. Below is the multiple cloning site(MCs)of the plasmid vector pUC 18 and the N-terminal and C-terminal sequence of protein X. Note that the MCS constitutes a part of the Lacz open reading frame. Suppose that you are going to clone the protein X gene into pUC18, so that your target gene is transcribed under the control of lacz promoter, and translated with the lacz gene to produce a fusion protein. You are requested to use BamHI and Pstl to the clone X gene, please add these restriction sites on the corresponding position of the X gene Remember to maintain the read ing frame of the X gene with the lacz gene (1)MCS of pUC18 Ecori Sacl Kpnl Smal BamHI Xbal Sa Pstl ACGAATTCG AGC TCG GTA CCC GGG GAT CCT CTA GAGTCG ACC TGC AGG CAT GCA Thr Asn SerSer Ser Val Pro Gly Asp Pro Leu Glu Ser Thr Cys Arg HisAla (2)N-terminal sequence of X gene ATG ACC CCU CAU AAC GGC GAC. (3)C-terminal sequence of X gene.. GAUAGU ACAGCU GCCAAGTAA … Asp Ser Thr Ala Ala Lysproportional to the number of genes contained within that genome. In other words, a genome that is 10 times as big will contains approximately 10 times as many protein coding genes. 1) True 2) False 13. HeLa cells, derived from a human cervical carcinoma, are able to propagate indefinitely in culture and are therefore known as a(n): 1) tissue culture. 2) tumor. 3) transgenic cell line. 4) immortalized cell line. 14. E. coli cells are smaller than yeast cells. 1) True 2) False 15. Which of the following domains is not a DNA binding domain 1) Proline-rich domains 2) Helix-turn helix domains 3) Zinc finger domains 4) Basic domains 16. The aminoacyl-tRNA synthetases distinguish between about 40 different shaped tRNA molecules in the cells. 1) True 2) False PART III: SHORT QUESTIONS (8 points each) 1. How do bacterial replication start and accomplished. Remember to include the proteins/enzymes and important DNA sequence involved in this process. 2. Design experiments to clone a yeast gene and express this gene in yeast. 3. Below is the multiple cloning site (MCS) of the plasmid vector pUC18 and the N-terminal and C-terminal sequence of protein X. Note that the MCS constitutes a part of the LacZ open reading frame. Suppose that you are going to clone the protein X gene into pUC18, so that your target gene is transcribed under the control of LacZ promoter, and translated with the LacZ gene to produce a fusion protein. You are requested to use BamHI and PstI to the clone X gene, please add these restriction sites on the corresponding position of the X gene. Remember to maintain the reading frame of the X gene with the LacZ gene 4. (1) MCS of pUC18 EcoRI SacI KpnI SmaI BamHI XbaI SalI PstI ACGAAT TCGAGC TCG GTA CCC GGG GAT CCT CTA GAG TCG ACC TGC AGG CAT GCA Thr Asn Ser Ser Ser Val Pro Gly Asp Pro Leu Glu Ser Thr Cys Arg His Ala (2) N-terminal sequence of X gene. ATG ACC CCU CAU AAC GGC GAC… Met Thr Pro His Asn Gly Asp… (3) C-terminal sequence of X gene. …GAU AGU ACA GCU GCC AAG TAA … Asp Ser Thr Ala Ala Lys Lac Z