1) transcription of ribosomal RNA 2) transcription of transfer RNa and other small RNa species 3) transcription of messenger RNA 4) initiation of Okazaki fragment synthesis in DNA replication 5. Restriction enzymes can cleave Dna that is either single-stranded or double-stranded as long as it contains the appropriate recognition site 1)True 2)False 6. Information about the sequence of the coding region of a gene is best obtained from 1) aYaC clone 2) 3) a cDNA clone 4) the protein 7. A chromatography method that can be used specifically to purify proteins based on their 1) gel filtration chromatography 2) ion-exchange chromatography 3) DNA affinity chromatography 4) antibody aff unity chromatography 8. A nonsense mutation is a change in the dNa sequence that results 1) a small deletion or insertion 2) an amino acid change in the protein encoded by the gene 3) a premature stop codon 4) all of the above 9. A protein complex involved in degradation of proteins within the cell is known as the 1) ubiquitin/proteasome system 2) molecular chaperone 3 cha onin 4) ribosome 5) Krebs/TCa cycle 10. binds to the repressor and turn on the transcription of the structural genes in the I)CAMP 3)allolactose 4)CRP 11. Which of the following RNA species is involved in degradation of the mRNA containing complementary sequence 1)miRNA 2)SIRNA 3)tRNA 4)5S RNA 12. The genome sequencing projects are confirming the theory that genome size is directly1) transcription of ribosomal RNA. 2) transcription of transfer RNA and other small RNA species. 3) transcription of messenger RNA. 4) initiation of Okazaki fragment synthesis in DNA replication. 5. Restriction enzymes can cleave DNA that is either single-stranded or double-stranded, as long as it contains the appropriate recognition site. 1) True 2) False 6. Information about the sequence of the coding region of a gene is best obtained from: 1) a YAC clone. 2) a genomic clone. 3) a cDNA clone. 4) the protein. 7. A chromatography method that can be used specifically to purify proteins based on their charge is: 1) gel filtration chromatography. 2) ion-exchange chromatography. 3) DNA affinity chromatography. 4) antibody affinity chromatography. 8. A nonsense mutation is a change in the DNA sequence that results in: 1) a small deletion or insertion. 2) an amino acid change in the protein encoded by the gene. 3) a premature stop codon. 4) all of the above. 5) none of the above. 9. A protein complex involved in degradation of proteins within the cell is known as the: 1) ubiquitin/proteasome system. 2) molecular chaperone. 3) chaperonin. 4) ribosome. 5) Krebs/TCA cycle. 10. ___binds to the repressor and turn on the transcription of the structural genes in the Lac operon. 1) cAMP 2) lactose 3) allolactose 4) CRP 11. Which of the following RNA species is involved in degradation of the mRNA containing complementary sequence 1) miRNA 2) siRNA 3) tRNA 4) 5S RNA 5) U3 snRNA 12. The genome sequencing projects are confirming the theory that genome size is directly