Planta a 0 Rosette leaves at bolting 60 40 20 0 NC 教料 #3 Opening of first flower 100 80 60 40 NC #1 #3 20 NC #1 #3 2.5 1.5 AtFLC AtTEM1 AtSPL15 2.0 1.0 3 5 2 0.5 0.5 0.0 00 NC #1 #3 NC 1 #3 NC 1.5 2.5 AtSOC1 AtMAF2 2.0 1.0 "S 1.0 0.5 0.5 0.0 0.0- NC #1 #3 NC #1 #3 Fig.4 Overexpression of BcFLC2 in Arabidopsis.a The NC and sent the standard deviation of the mean number of 30 plants for each 35S:BcFLC2-GFP #and #3 plants grown in a chamber (16 h line.**indicates significant differences from control (P<0.01).d light/8 h dark photoperiod at 22 C/18 C).Scale bars=1.5 cm. Expression analysis of predicted downstream genes in the NC and Rosette leaf number at bolting (b)and opening of first flower (c)in 35S:BcFLC2-GFP #I and #3 plants the NC and 35S:BcFLC2-GFP #and #3 plants.Error bars repre- ②SpringerPlanta 1 3 Fig. 4 Overexpression of BcFLC2 in Arabidopsis. a The NC and 35S:BcFLC2-GFP #1 and #3 plants grown in a  chamber (16  h light/8  h dark photoperiod at 22  °C/18  °C). Scale bars=1.5  cm. Rosette leaf number at bolting (b) and opening of frst fower (c) in the NC and 35S:BcFLC2-GFP #1 and #3 plants. Error bars repre￾sent the standard deviation of the mean number of 30 plants for each line. ** indicates signifcant diferences from control (P<0.01). d Expression analysis of predicted downstream genes in the NC and 35S:BcFLC2-GFP #1 and #3 plants