Chapter 3 Exploring Proteins and Proteomes Matching Questions Use the following to answer questions 1-10: Choose the correct answer from the list below.Not all of the answers will be used a)HPLC b)specific activity c)MALDI-TOF mass spectrum d)zonal centrifugation e)nascent f)SDS g)epitope h)Svedberg i)immunoglobulin j)centrifugation k)overlap peptides 1)affinity chromatography 1 The ratio of enzyme activity relative to total protein is called Ans:b Section:3.1 2 The first step in protein purification from a homogenate is usually Ans:j Section:3.1 A type of purification that is based on the attraction of the protein for a particular chemical group Ans:I Section:3.1 can be added prior to gel electrophoresis to denature the proteins. Ans:f Section:3.1 5 Sedimentation coefficients are described as units. Ans:h Section:3.1Chapter 3 Exploring Proteins and Proteomes Matching Questions Use the following to answer questions 1-10: Choose the correct answer from the list below. Not all of the answers will be used. a) HPLC b) specific activity c) MALDI-TOF mass spectrum d) zonal centrifugation e) nascent f) SDS g) epitope h) Svedberg i) immunoglobulin j) centrifugation k) overlap peptides l) affinity chromatography 1 The ratio of enzyme activity relative to total protein is called ____________. Ans: b Section: 3.1 2 The first step in protein purification from a homogenate is usually ____________. Ans: j Section: 3.1 3 ____________ A type of purification that is based on the attraction of the protein for a particular chemical group. Ans: l Section: 3.1 4 ____________ can be added prior to gel electrophoresis to denature the proteins. Ans: f Section: 3.1 5 Sedimentation coefficients are described as ____________ units. Ans: h Section: 3.1