BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Piezoelectric, 9 Bs如 quaitzerystlmicrobslan ce detecion 0cyB0。减ad叫食qban。adw d加 mation induced as dipoles in crystals如a! with d rec ion ofelechict Dm10100mA呲吗 infreq range d1-10M oa Bindng of analye to surace changes mass of crystal and ater osc aion frequency Figure below from: www-bond. chem. monash. edu. au/theses/ Graeme%/20Snook/Chapterl. pdf Piezoelectric detection Quartz crystal microbalance Analyte solution B http://ww.g-sense.com/main.gcmdtech.html Lecture 19-BiosensorsBEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Piezoelectric8,9 ƒ Based on quartz crystal microbalance detection Based on quartz crystal microbalance detection o Crystal is oscillated at a defined frequency by an oscillating applied voltage ƒ Shear deformation induced as dipoles in crystal seek to align with direction of electric field Shear deformation induced as dipoles in crystal seek to align with direction of electric field ƒ Deformation typically 10-100 nm for AT-cut crystals operating in freq. range of 1-10 MHz ystals operating in freq. range of 1-10 MHz oBinding of analyte to surface changes mass of crystal and alter oscillation frequency of crystal and alter oscillation frequency ƒ Figure below from : www-bond.chem.monash.edu.au/theses/ Graeme%20Snook/Chapter1.pdf Piezoelectric detection: Quartz crystal microbalance Analyte solution http://www.q-sense.com/main.qcmd_tech.html Lecture 19 – Biosensors