BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Calorimetric detection glucose-6-phosphate ADP pyruvate kinase H +NADH NADX Schematic diagram of a calorimetric biosensor. The sampl pyrus rence thermistor(e)and into the packed bed bioreactor(f, 1ml volume), containing the biocatalyst, where the reaction occurs. The change in temperature is determined by the thermistor(g)and the oxyger difference in the resistance, and hence temperature between the http://www.sbu.ac.uk/biology/enztech/calorimetric.html thermistors Lecture 19-BiosensorsBEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Calorimetric detection: QuickTime™ and a TIFF (LZW) decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. http://www.sbu.ac.uk/biology/enztech/calorimetric.html Schematic diagram of a calorimetric biosensor. The sample stream (a) passes through the outer insulated box (b) to the heat exchanger (c) within an aluminium block (d). From there, it flows past the reference thermistor (e) and into the packed bed bioreactor (f, 1ml volume), containing the biocatalyst, where the reaction occurs. The change in temperature is determined by the thermistor (g) and the solution passed to waste (h). External electronics (l) determines the difference in the resistance, and hence temperature, between the thermistors. Lecture 19 – Biosensors