6 基因突变检测原理和流程 ●●●● Enter Query Sequence Enter accession number(s),gi(s).or FASTA sequence(s) Clear Query subrange AAC GTC TTC CIT CTC TCT CTG TCA OCA CAC AGC丛GCAG丛ACT From New columns added to the To Description Table Now Click 'Select Columns'or 'Manage Columns'. Or,upload file 选挥文件未选择任何文件 Job Title Enter a descriptive title for your BLAST search Align two or more sequences Choose Search Set Database OStandard databases (nr etc.)OrRNA/ITS databasesGenomic+transcript databases Betacoronavirus Human genomic plus transcript (Human G+T)(160592 sequences) Exclude Models (XM/XP)Uncultured/environmental sample sequences Optional Limit to Sequences from type material Ootfonal Entrez Query You Create custom database Optional Enter an Entrez query to limit search Program Selection Optimize for Highly similar sequences(megablast) More dissimilar sequences (discontiguous megablast) .Somewhat similar sequences (blastn) Choose a BLAST algorithm 6 基因突变检测原理和流程