proliferate indefinitely when grown in culture.Mouse B lymphocytes and myeloma cells are fused by adding polyethylene glycol,which acts as a chemical glue.The result is a hybridoma,a cell that combines the antibody producing capability of a B lymphocyte with the myeloma cell's capacity to divide and reproduce virtually forever.A combination that assures a nearly endless source of antibodies.Among the numerous hybridomas formed will be a few cells producing the antibody of the appropriate specificity The desired cells are found and separated in a three-step process:selection, screening,and cloning.The first step is used to select only those cells that have fused to form a hybridoma;all unfused myeloma and spleen cells are eliminated.Hybridoma cells are screened by one of various immunological procedures to identify those hybridomas that are producing antibody molecules of the Figure 11. Figure 12 Figure 13. Figure 14. Figure 15. Figure 16 Figure 17A desired specificity.At this point,the hybridoma cells are usually growing as small colonies,in separate wells of 96 well culture plates.The final step is to ensure that all cells in the culture are producing the sameproliferate indefinitely when grown in culture. Mouse B lymphocytes and myeloma cells are fused by adding polyethylene glycol, which acts as a chemical glue. The result is a hybridoma, a cell that combines the antibody producing capability of a B lymphocyte with the myeloma cell's capacity to divide and reproduce virtually forever. A combination that assures a nearly endless source of antibodies. Among the numerous hybridomas formed will be a few cells producing the antibody of the appropriate specificity. The desired cells are found and separated in a three-step process: selection, screening, and cloning. The first step is used to select only those cells that have fused to form a hybridoma; all unfused myeloma and spleen cells are eliminated. Hybridoma cells are screened by one of various immunological procedures to identify those hybridomas that are producing antibody molecules of the Figure 11. Figure 12. Figure 13. Figure 14. Figure 15. Figure 16 Figure 17A. desired specificity. At this point, the hybridoma cells are usually growing as small colonies, in separate wells of 96 well culture plates. The final step is to ensure that all cells in the culture are producing the same