phoa gene fusions带 Export and activity restored by fusing in frame a restriction fragment to truncated phoa gene; i.e lacking signal sequences No AUG start codon No ribosome binding site Restriction fragment has portions of NH2 terminal genes encoding signal sequences of heterologous proteins, such as OmpF or LamB BIOL 533 Lecture 4BIOL 533 11 Lecture 4 phoA Gene Fusions • Export and activity restored by fusing in frame a restriction fragment to truncated phoA gene; i.e., lacking signal sequences – No AUG start codon – No ribosome binding site • Restriction fragment has portions of NH2 - terminal genes encoding signal sequences of heterologous proteins, such as OmpF or LamB