TnphoA Transposon Created Tnphoa that randomly inserts phoa into target genes Hybrid proteins display alkaline phosphatase activity only if target gene encodes a periplasmic outer membrane or extracellular protein BIOL 533 Lecture 4BIOL 533 12 Lecture 4 TnphoA Transposon • Created TnphoA that randomly inserts phoA into target genes • Hybrid proteins display alkaline phosphatase activity only if target gene encodes a periplasmic outer membrane or extracellular protein