Laboratory Diagnosis ■ Virus isolation Mainstay of diagnosis of poliovirus infection poliovirus can be readily isolated from throat swabs faeces, and rectal swabs, but rarely from the csF Can be readily grown and identified in cell culture Requires molecular techniques to differentiate between the wild type and the vaccine type Serology Very rarely used for diagnosis since cell culture is efficient. Occasionally used for immune status screening for immunocompromised individualsLaboratory Diagnosis ◼ Virus Isolation ◆ Mainstay of diagnosis of poliovirus infection ◆ poliovirus can be readily isolated from throat swabs, faeces, and rectal swabs, but rarely from the CSF ◆ Can be readily grown and identified in cell culture ◆ Requires molecular techniques to differentiate between the wild type and the vaccine type ◼ Serology ◆ Very rarely used for diagnosis since cell culture is efficient. Occasionally used for immune status screening for immunocompromised individuals