Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S Gerstein opyright◎2001 ISBNS:0-471-37972-7( Paper);0-47 (Electronic) Nucleotides, Oligonucleotides, and Polynucleotides Alan S Gerstein 268 Nomenclature: De facto and Du jour What Makes a Nucleotide pure? 269 Are Solution Nucleotides Always More Pure Than Lyophilized Nucleotides 269 Are Solution Nucleotides More Stable Than Lyophilized Nucleotides? 270 Does Your Application Require Extremely Pure Nucleotides? 272 How Can You Monitor Nucleotide Purity and Degradation 272 The author would like to thank Anita Gradowski of Pierce Milwaukee for contributing such thorough and helpful information regarding the preparation of nucleotide solutions. Special thanks also to Cica Minetti and David Remeta of Rutgers University for discussing a method to calculate the extinction coefficient of an oligonucleotide. The contributions to this chapter by Howard Coyer and Thomas Tyre, also of Pierce Milwaukee are too numerous to list 267267 10 Nucleotides, Oligonucleotides, and Polynucleotides Alan S. Gerstein Nucleotides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268 Nomenclature: De facto and Du jour . . . . . . . . . . . . . . . . . . 268 What Makes a Nucleotide Pure? . . . . . . . . . . . . . . . . . . . . . . . 269 Are Solution Nucleotides Always More Pure Than Lyophilized Nucleotides?. . . . . . . . . . . . . . . . . . . . . . . . . . . . 269 Are Solution Nucleotides More Stable Than Lyophilized Nucleotides? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 270 Does Your Application Require Extremely Pure Nucleotides? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 272 How Can You Monitor Nucleotide Purity and Degradation?. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 272 The author would like to thank Anita Gradowski of Pierce Milwaukee for contributing such thorough and helpful information regarding the preparation of nucleotide solutions. Special thanks also to Cica Minetti and David Remeta of Rutgers University for discussing a method to calculate the extinction coefficient of an oligonucleotide. The contributions to this chapter by Howard Coyer and Thomas Tyre, also of Pierce Milwaukee, are too numerous to list. Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, Inc. ISBNs: 0-471-37972-7 (Paper); 0-471-22390-5 (Electronic)