How Should You Prepare, Quantitate, and Adjust the PH of Small and Large volumes of Nucleotides? 273 What ls the Effect of Thermocycling on Nucleotide Is There a difference between absorbance, A260, and Optical Density? 275 Why Do A260 Unit Values for Single-Stranded DNA and Oligonucleotides Vary in the Research Literature? 278 279 How Pure an ol Application? 279 What Are the Options for Quantitating Oligonucleotides 279 What ls the Storage Stability of Oligonucleotides? Your Vial of Oligonucleotide ls Empty, or ls It? 28 Synthetic Polynucleotides Is a Polynucleotide ldentical to an Oligonucleotide 28 How Are Polynucleotides Manufactured and How Might This Affect your research? 282 Would the World Be a Better Place If Polymer Length Never varied? Oligonucleotides Don't Suffer from Batch to Batch Size Variation. Why Not ow Many Micrograms of Polynucleotide Are in Your Vial? Is It possible to determine the Molecular Weight of a Polynucleotide? 285 What Are the Strategies for Preparing Polymer Solutions of Known concentration 285 Your Cuvette has a 10mm Path Length what Absorbance values Would be observed for the same Why Not Weigh out a portion of the polymer Instead..286 Solution If Your Cuvette Had a 5mm Path Length of Dissolving the Entire Contents of the Vial Is a Phosphate Group Present at the 5 End of ynthetic Nucleic Acid Polym ner What Are the Options for Preparing and Storing Solutions of Nucleic Acid Polymers? Bibliography NUCLEOTIDES Nomenclature: De facto and Du jour Lehninger (1975) provides a thorough discussion of prope nucleotide nomenclature and abbreviations. Unfortunately, 268 GersteinHow Should You Prepare, Quantitate, and Adjust the pH of Small and Large Volumes of Nucleotides? . . . . . . . . . . . 273 What Is the Effect of Thermocycling on Nucleotide Stability?. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 275 Is There a Difference between Absorbance, A260, and Optical Density? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 275 Why Do A260 Unit Values for Single-Stranded DNA and Oligonucleotides Vary in the Research Literature? . . . . . . 278 Oligonucleotides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279 How Pure an Oligonucleotide Is Required for Your Application?. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279 What Are the Options for Quantitating Oligonucleotides? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279 What Is the Storage Stability of Oligonucleotides?. . . . . . . . 280 Your Vial of Oligonucleotide Is Empty, or Is It? . . . . . . . . . . . 281 Synthetic Polynucleotides. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 281 Is a Polynucleotide Identical to an Oligonucleotide?. . . . . . . 281 How Are Polynucleotides Manufactured and How Might This Affect Your Research? . . . . . . . . . . . . . . . . . . . . . . . . . . 282 Would the World Be a Better Place If Polymer Length Never Varied? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 284 Oligonucleotides Don’t Suffer from Batch to Batch Size Variation. Why Not? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 284 How Many Micrograms of Polynucleotide Are in Your Vial? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 284 Is It Possible to Determine the Molecular Weight of a Polynucleotide? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 285 What Are the Strategies for Preparing Polymer Solutions of Known Concentration? . . . . . . . . . . . . . . . . . . . . . . . . . . 285 Your Cuvette Has a 10mm Path Length. What Absorbance Values Would Be Observed for the Same Solution If Your Cuvette Had a 5mm Path Length? . . . . . 286 Why Not Weigh out a Portion of the Polymer Instead of Dissolving the Entire Contents of the Vial?. . . . . . . . . . 287 Is a Phosphate Group Present at the 5¢ End of a Synthetic Nucleic Acid Polymer? . . . . . . . . . . . . . . . . . . . . . 287 What Are the Options for Preparing and Storing Solutions of Nucleic Acid Polymers? . . . . . . . . . . . . . . . . . . 287 Bibliography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 288 NUCLEOTIDES Nomenclature: De facto and Du jour Lehninger (1975) provides a thorough discussion of proper nucleotide nomenclature and abbreviations. Unfortunately, 268 Gerstein