Question 1 A)You infected mice with mouse mammary tumor virus(a retrovirus). After a period of time, most infected mice had developed breast tumors whereas uninfected mice did not. You isolated cell lines from over 50 independent tumors. You demonstrated that all of these lines had virus integrations in the same chromosomal location. Can one conclude that the virus integrates into cellular dNA at only one site? Explain. B)The ras oncogene is involved in a variety of human and animal cancers. DNA was isolated from a number of normal and cancerous tissues -Cellular dna was digested with ecori Digested DNA was separated by gel electrophoresis and transfered to a nitrocellulose membrane The membrane was probed with the radioactive labelled cloned ras DNA and then the membrane was exposed to x-ray film The resulting autoradiograph is shown below 1)white blood cells from a healthy human 2)human lymphoma cells(cancerous) 3)human bladder carcinoma cells(cancerous) 4)human sarcoma cells(cancerous) 5)blood from a healthy mouse 6)mouse myeloma cells(cancerous) 3 10 kb 5 kb 1 kb more than 2 copies/genome 2 copies/genome 1 copies/genome2 Question 1 A) You infected mice with mouse mammary tumor virus (a retrovirus). After a period of time, most infected mice had developed breast tumors, whereas uninfected mice did not. You isolated cell lines from over 50 independent tumors. You demonstrated that all of these lines had virus integrations in the same chromosomal location. Can one conclude that the virus integrates into cellular DNA at only one site? Explain. B) The ras oncogene is involved in a variety of human and animal cancers. DNA was isolated from a number of normal and cancerous tissues. -Cellular DNA was digested with EcoRI. -Digested DNA was separated by gel electrophoresis and transfered to a nitrocellulose membrane. -The membrane was probed with the radioactive labelled cloned ras DNA and then the membrane was exposed to x-ray film. -The resulting autoradiograph is shown below. 1) white blood cells from a healthy human 2) human lymphoma cells (cancerous) 3) human bladder carcinoma cells (cancerous) 4) human sarcoma cells (cancerous) 5) blood from a healthy mouse 6) mouse myeloma cells (cancerous) 1 2 3 4 5 6 10 kb 5 kb 1 kb more than 2 copies/genome 2 copies/genome 1 copies/genome