Mechanisms of heat induced damage Cytoplasmic and nuclear protein denaturation DNA and RNA changes and cellular membrane function change Tissue necrosis,coagulation and vaporization of tissue Damage degree function of tissue temperature and treatment timeMechanisms of heat induced damage • Cytoplasmic and nuclear protein denaturation • DNA and RNA changes and cellular membrane function change • Tissue necrosis, coagulation and vaporization of tissue Damage degree : function of tissue temperature and treatment time