Absorption and fluorescence spectrum of GFp 6 &2 WAVELENGTH(nm) FIGURE 2: Absorption spectra of GFPb(1) and GFPa(2), fluores cence emission spectrum of GFPb excited by 400 nm (3), and fluorescence excitation spectrum of GFPb measured by emission at 508 nm(4). Protein concentrations were 0.85 mg/ml for(1)and (2), and 0.02 mg/ml for(3)and(4), all in 10 mM sodium phosphate buffer(pH 7.0) at 25. Fluorescence spectra were measured in a 3-mm cell VUV UVC UVB UVA 100 200 300 400 500 60o Morise, H, Shimomura, O; et al Biochemistry 1974, 13, 2656Morise, H.; Shimomura, O.; et.al Biochemistry 1974, 13, 2656 Absorption and fluorescence spectrum of GFP