ROSETTA search al algorithm Monte Carlo/ simulated Annealing Structures are assembled from fragments by Begin with a fully extended chain Randomly replace the conformation of one 9 residue segment with the conformation of one of its neighbors in the library Evaluate the move: Accept or reject based on an energy function Make another random move After a prescribed number of cycles switch to 3- residue fragment movesROSETTA search algorithm Monte Carlo/Simulated Annealing • Structures are assembled from fragments by: – Begin with a fully extended chain – Randomly replace the conformation of one 9 residue segment with the conformation of one of its neighbors in the library – Evaluate the move: Accept or reject based on an energy function – Make another random move… – After a prescribed number of cycles, switch to 3- residue fragment moves