ROSETTA fragment libraries Remove all homologs of the protein to be modeled(25% sequence identity) For each 9 residue segment in the target use sequence similarity and secondary structure similarity(compare predicted secondary stucture for target to fragment secondary structure) to select w 25 fragments Because secondary structure is influenced by tertiary structure, ensure that the fragments span different secondary structures The extent to which the fragments cluster around a consensus structure is correlated with how good a model the fragment is likely to be for the target LSERTVARS①eROSETTA fragment libraries • Remove all homologs of the protein to be modeled (>25% sequence identity) • For each 9 residue segment in the target, use sequence similarity and secondary structure similarity (compare predicted secondary stucture for target to fragment secondary structure) to select ~25 fragments • Because secondary structure is influenced by tertiary structure, ensure that the fragments span different secondary structures • The extent to which the fragments cluster around a consensus structure is correlated with how good a model the fragment is likely to be for the target LSERTVARS