(7) The phosphoglycerate kinase catalyzes the direct transfer of the anhydride phosphate in 1, 3-BPG to an aDP to generate an atp this is called the substrate-level phosphorylation;1, 3-BPG is a high energy intermediate that leads to atp formation (8) The phosphoglycerate mutase catalyzes the shift of phosphoryl group on 3 phosphoglycerate from c-3 to c-2 1 2 3 bisphosphoglycerate is both a coenzyme for the mutase and an intermediate for the reaction（7）The phosphoglycerate kinase catalyzes the direct transfer of the anhydride phosphate in 1,3-BPG to an ADP to generate an ATP; this is called the substrate-level phosphorylation; 1,3-BPG is a high energy intermediate that leads to ATP formation. （8）The phosphoglycerate mutase catalyzes the shift of phosphoryl group on 3- phosphoglycerate from C-3 to C-2; 2,3- bisphosphoglycerate is both a coenzyme for the mutase and an intermediate for the reaction