SenemeaombesgnhienmS28rploieRpense6ceny-eventhoughts t can be alleviated by recording spectra in 90%D.O/10%H.O to reduce the proton density in the deuterated protein This will significantly reduce the S/N of the spectra. n a 98%8 0.6 0.4 03 0 10 20 30 50 60 Residue number erauon is ne adiation and to reduce spin diffusion effects ○Irradiated Affected X Unaffected Ramos et al(2000)JACS12211311-11314 Spin diffusion between NHs in protein 1 happens efficiently - even though it is deuterated there is a high density of protons. It can be alleviated by recording spectra in 90% D2O/10% H2O to reduce the proton density in the deuterated protein This will significantly reduce the S/N of the spectra. 10% H2O 90% H2O Deuteration is necessary to provide a window for irradiation and to reduce spin diffusion effects Ramos et al (2000) JACS 122 11311-11314 X Irradiated Affected Unaffected