分析化学 第34卷 20 Wei Y, Cao C, J in R, Mirkin C A Science, 2002, 297: 15361540 21 Bao Y P, HuberM, Wei T, Marla SS, Strhoff J J, Muller UR Nucleic A cids Res, 2005, 33 (2): e15 22 Francoisa P. Benta M. Vaudauxa P. Schrenzel J.J M icpbiol Meth. 2003. 55: 755-762 J. LIu h 24 Wang Y, Pang D, Zhang Z, Zheng H, Cao J, Shen I J Med VinDI, 2003, 70: 205-211 5 Ramakrishnan R, Buck ingham W, DamanusM, Gieser L, Klein K, Kunkel G, Prokhomva A, R iccelli P. Clin Chen 26 HuberM, Wei T, Muller U R, Lefebvre P A, Marla SS, Bao Y P. Nucleic A cids Res, 2004, 32(18): e137 7 LiangR, Liw, Li Y, Tan C, LiJ, Jin Y, Ruan k Nucleic A cids Res, 2005, 33 (2): el7 28 StonhoffJ J, Marla S S, Bao P, Hagenow S, Mehta H, Lucas A, Garmella V, Patno T, Buck ingham W, Cork W, Muller UR Biasens B ioelectron, 2004. 19: 875-883 29 JiM. Hou P. LiS. Hen. Lu z Clin Chin. Acta. 2004. 342: 145-153 30 Han A, Dufva M, Belleville E, Christensen CB V. Lab Chip, 2003, 3(4): 329-332 31 Guo H, Zhang J, Yang D, Xiao P, He N. Colloid Surface B, 2005, 40: 195-198 32 MandalM, Kundu S, Ghosh S K, Panigrahi S, Sau T K, Yusuf SM, Pal J Colloid In terface Sci, 2005, 286: 187-194 33 Nam J, Thaxton C, Mirkin C A. Science, 2003, 301: 1884-1886 34 Georganopoubu D G, Chang L, Nam J, Thaxton C S, Mufson E J, Klen W L, Mirkin C A PNAS, 2005, 102(7) 2273~2276 35 Pang j ian tao逄键涛), Zhang ili(张敏丽), Wen Siyuan(文思远), W ang Shengqi(升启). Bulletin of the A cadeny fM ilitary M ical Sciences((军事医学科学院院刊),2005,29(3):223~226 36 Yarez-Sedep P. Pingarron J. Anal B ional Chen. 2005. 382: 884--886 37 Park S. Tatn TA. Mirkin C A Science. 2002. 295: 1503-1506 38 Mitchell JS, Wu Y, Cook C J, Main L Anal B lochen, 2005, 343: 125-135 39 Liu T, Tang J, J iang L B lochen. B iophys Conn 40 A slan K, Lakow icz J R, Geddes C D. Anal B lochan, 2004, 330: 145-155 41 Dubertret B. Cakme M. L ibchabera. Nat B iotechnoI. 2001. 19: 365-370 D eve lopm en t of the a ggrega tion of Gold Nan particles and Nanoprobe M icroarrays Technolog ies Pang j antao, wen Siyuan, W ang sheng r Institute of Radiation Med icine, A caden y of M ilitary M ed ical Sciences Beijing 100850) A bstract Gold nanoparticle(GNP) probes have abused more and more interest recently. Here we review the app lication and advancement of gnP p robes in bpassay, which focuses on the aggregation of GNP and m ic array de tecton The concemed electrochem ical and other methods have also been discussed 41 papers ware cited Keywords Gold nanopartic le, m ic marray, bioassay, review (Receied 10 August 2005: accep ted 3 December 2005) g1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreservedhttp://www.cnki.net20 W ei Y, Cao C, Jin R, M irkin C A. Science, 2002, 297: 1536～1540 21 Bao Y P, HuberM, W ei T, Marla S S, Storhoff J J, Müller U R. N ucleic Acids Res. , 2005, 33 (2) : e15 22 Francoisa P, Bentoa M, Vaudauxa P, Schrenzel J. J. M icrobiol. M eth. , 2003, 55: 755～762 23 W ang Y, Shen J, L iu H. J. V irolM ethods, 2004, 121: 79～84 24 W ang Y, Pang D, Zhang Z, Zheng H, Cao J, Shen J. J. M ed. V irol. , 2003, 70: 205～211 25 Ramakrishnan R, Buckingham W , DomanusM, Gieser L, Klein K, Kunkel G, Prokhorova A, Riccelli P. Clin. Chem. , 2004, 50 (10) : 1949～1952 26 HuberM, W ei T, Müller U R, Lefebvre P A, Marla S S, Bao Y P. N ucleic Acids Res. , 2004, 32 (18) : e137 27 L iang R, L iW , L i Y, Tan C, L i J, Jin Y, Ruan K. N ucleic Acids Res. , 2005, 33 (2) : e17 28 Storhoff J J, Marla S S, Bao P, Hagenow S, Mehta H, Lucas A, Garimella V, Patno T, Buckingham W , Cork W , Müller U R. B iosens. B ioelectron. , 2004, 19: 875～883 29 JiM, Hou P, L i S, He N , Lu Z. Clin. Chim. Acta, 2004, 342: 145～153 30 Han A, Dufva M, Belleville E, Christensen C B V. Lab. Chip. , 2003, 3 (4) : 329～332 31 Guo H, Zhang J, Yang D, Xiao P, He N. Colloid Surface B , 2005, 40: 195～198 32 MandalM, Kundu S, Ghosh S K, Panigrahi S, Sau T K, Yusuf SM, Pal. J. Colloid Interface Sci. , 2005, 286: 187～194 33 Nam J, Thaxton C, M irkin C A. Science, 2003, 301: 1884～1886 34 Georganopoulou D G, Chang L, Nam J, Thaxton C S, Mufson E J, Klein W L, M irkin C A. PNAS , 2005, 102 ( 7) : 2273～2276 35 Pang Jiantao (逄键涛 ) , ZhangM inli(张敏丽 ) ,W en Siyuan (文思远 ) , W ang Shengqi(王升启 ). B ulletin of the Academ y of M ilitary M edical Sciences(军事医学科学院院刊 ) , 2005, 29 (3) : 223～226 36 YáÌez2SedeÌo P, Pingarrón J. Anal. B ioanal. Chem. , 2005, 382: 884～886 37 Park S, Taton T A, M irkin C A. Science, 2002, 295: 1503～1506 38 M itchell J S, W u Y, Cook C J, Main L. Anal. B iochem. , 2005, 343: 125～135 39 L iu T, Tang J, Jiang L. B iochem. B iophys. Comm un. , 2004, 313: 3～7 40 A slan K, Lakowicz J R, Geddes C D. Anal. B iochem. , 2004, 330: 145～155 41 Dubertret B, Calame M, L ibchaber A J. N at. B iotechnol. , 2001, 19: 365～370 D evelopm en t of the Aggrega tion of Gold Nanoparticles and Nanoprobe2M icroarrays Technolog ies Pang Jiantao, W en Siyuan, W ang Shengqi 3 ( Institute of Radiation M edicine, Academ y of M ilitary M edical Sciences, B eijing 100850) Abstract Gold nanoparticle ( GNP) p robes have aroused more and more interest recently. Here we review the app lication and advancement of GNP p robes in bioassay, which focuses on the aggregation of GNP and m icroarray detection. The concerned electrochem ical and other methods have also been discussed. 41 papers ware cited. Keywords Gold nanoparticle, m icroarray, bioassay, review (Received 10 August 2005; accep ted 3 December 2005) 888 分 析 化 学 第 34卷