Reciprocating Pump (Fig 28-6) Column 凵Pulse Motor Seal damper Ball check valves Reciprocating Solvent Up to 10,000 psi,small internal volumes ·Produces pulsation Sample injection(Fig 28-7): Load sample Inject sample 00p column From pump From pump ·Similar to FIA,GC Introduce small sample (0.1-100 uL)without depressurization Microsyringe/septum system (only <1500 psi) CEM 333 page 16.3Reciprocating Pump (Fig 28-6): • Up to 10,000 psi, small internal volumes • Produces pulsation Sample injection (Fig 28-7): • Similar to FIA, GC • Introduce small sample (0.1-100 mL) without depressurization • Microsyringe/septum system (only <1500 psi) CEM 333 page 16.3