the first direct measurement,recognizing their agency in the topic position may well be appropriate. 2.Since "dG and "dC"become the old information in the second sentence,keep them up front in the topic position. 3.Since "triisopropylsilyl groups"is new and important information here,create for it a stress position. 4."Triisopropylsilyl groups"then becomes the old information of the clause in which its effects are described;place it in the topic position of this clause. 5.Alert the reader to expect the arrival of two distinct effects by using the flag word"both.""Both" notifies the reader that two pieces of new information will arrive in a single stress position. Here is a partial revision based on these decisions: We have directly measured the enthalpy of hydrogen bond formation between the nucleoside bases 2'deoxyguanosine(dG)and 2'deoxycytidine(dC).dG and dC were derivatized at the 5'and 3 hydroxyls with triisopropylsilyl groups;these groups serve both to solubilize the nucleosides in non-aqueous solvents and to prevent the ribose hydroxyls from forming hydrogen bonds.From isoperibolic titration measurements,the enthalpy of dC:dG base pair formation is-6.65+0.32 kcal/mol The outlines of the experiment are now becoming visible,but there is still a major logical gap.After reading the second sentence,we expect to hear more about the two effects that were important enough to merit placement in its stress position.Our expectations are frustrated,however,when those effects are not mentioned in the next sentence:"From isoperibolic titration measurements,the enthalpy of dC:dG base pair formation is-6.65+0.32 kcal/mol."The authors have neglected to explain the relationship between the derivatization they performed(in the second sentence)and the measurements they made(in the third sentence).Ironically,that is the point they most wished to make here. At this juncture,particularly astute readers who are chemists might draw upon their specialized knowledge,silently supplying the missing connection.Other readers are left in the dark.Here is one version of what we think the authors meant to say,with two additional sentences supplied from a knowledge of nucleic acid chemistry: We have directly measured the enthalpy of hydrogen bond formation between the nucleoside bases 2'deoxyguanosine (dG)and 2'deoxycytidine(dC).dG and dC were derivatized at the 5'and 3' hydroxyls with triisopropylsiyl groups;these groups serve both to solubilize the nucleosides in non-aqueous solvents and to prevent the ribose hydroxyls from forming hydrogen bonds. Consequently,when the derivatized nucleosides are dissolved in non-aqueous solvents,hydrogen bonds form almost exclusively between the bases.Since the interbase hydrogen bonds are the only bonds to form upon mixing,their enthalpy of formation can be determined directly by measuring the enthalpy of mixing.From our isoperibolic titration measurements,the enthalpy of dG:dC base pair formation is-6.65+0.32 kcal/mol. Each sentence now proceeds logically from its predecessor.We never have to wander too far into a sentence without being told where we are and what former strands of discourse are being continued And the "measurements"of the last sentence has now become old information,reaching back to the "measured directly"of the preceding sentence.(It also fulfills the promise of the "we have directly measured"with which the paragraph began.)By following our knowledge of reader expectations,wethe first direct measurement, recognizing their agency in the topic position may well be appropriate.) 2. Since "dG and "dC" become the old information in the second sentence, keep them up front in the topic position. 3. Since "triisopropylsilyl groups" is new and important information here, create for it a stress position. 4. "Triisopropylsilyl groups" then becomes the old information of the clause in which its effects are described; place it in the topic position of this clause. 5. Alert the reader to expect the arrival of two distinct effects by using the flag word "both." "Both" notifies the reader that two pieces of new information will arrive in a single stress position. Here is a partial revision based on these decisions: We have directly measured the enthalpy of hydrogen bond formation between the nucleoside bases 2’deoxyguanosine (dG) and 2’deoxycytidine (dC). dG and dC were derivatized at the 5’ and 3’ hydroxyls with triisopropylsilyl groups; these groups serve both to solubilize the nucleosides in non-aqueous solvents and to prevent the ribose hydroxyls from forming hydrogen bonds. From isoperibolic titration measurements, the enthalpy of dC:dG base pair formation is -6.65±0.32 kcal/mol. The outlines of the experiment are now becoming visible, but there is still a major logical gap. After reading the second sentence, we expect to hear more about the two effects that were important enough to merit placement in its stress position. Our expectations are frustrated, however, when those effects are not mentioned in the next sentence: "From isoperibolic titration measurements, the enthalpy of dC:dG base pair formation is -6.65±0.32 kcal/mol." The authors have neglected to explain the relationship between the derivatization they performed (in the second sentence) and the measurements they made (in the third sentence). Ironically, that is the point they most wished to make here. At this juncture, particularly astute readers who are chemists might draw upon their specialized knowledge, silently supplying the missing connection. Other readers are left in the dark. Here is one version of what we think the authors meant to say, with two additional sentences supplied from a knowledge of nucleic acid chemistry: We have directly measured the enthalpy of hydrogen bond formation between the nucleoside bases 2’deoxyguanosine (dG) and 2’deoxycytidine (dC). dG and dC were derivatized at the 5’ and 3’ hydroxyls with triisopropylsiyl groups; these groups serve both to solubilize the nucleosides in non-aqueous solvents and to prevent the ribose hydroxyls from forming hydrogen bonds. Consequently, when the derivatized nucleosides are dissolved in non-aqueous solvents, hydrogen bonds form almost exclusively between the bases. Since the interbase hydrogen bonds are the only bonds to form upon mixing, their enthalpy of formation can be determined directly by measuring the enthalpy of mixing. From our isoperibolic titration measurements, the enthalpy of dG:dC base pair formation is -6.65±0.32 kcal/mol. Each sentence now proceeds logically from its predecessor. We never have to wander too far into a sentence without being told where we are and what former strands of discourse are being continued. And the "measurements" of the last sentence has now become old information, reaching back to the "measured directly" of the preceding sentence. (It also fulfills the promise of the "we have directly measured" with which the paragraph began.) By following our knowledge of reader expectations, we