中国农业科学2009,42(1)312-317 doi:10.3864/issn.0578-1752.2009.01.040 恩诺沙星及其代谢产物在奶牛乳中的消除规律 廖洁丹2,李守军,丁焕中',刘文字,余静贤',张嘉慧',刘雅红 (华南农业大学兽医学院,广州510642:2佛山科学技术学院,广东佛山528000 摘要:【目的】研究乳房炎奶牛单剂量乳房灌注1%恩诺沙星(50m1/头)后乳中药物浓度的变化规律。【方 法】采用HPLC法测定乳中恩诺沙星及其代谢产物环丙沙星的浓度,用统计矩原理处理药物浓度一时间数据。【结 果】在给药区恩诺沙星和代谢产物环丙沙星均于给药后2h左右乳中浓度达到最高值,分别为(13.16±3.10)ugm1 和(2.79±0.94)μgmn1,然后逐渐下降;12h后检测不到恩诺沙星,24h已检测不到环丙沙星。对于非给药 区,恩诺沙星给药后1h左右乳中浓度达到最高值(0.19±0.02)ug"m1,然后逐渐下降;6h后检测不到恩诺沙 星,而代谢产物环丙沙星于给药后4h乳药浓度可达最高峰(0.51±0.07)ugm1,24h已检测不到乳药浓度。 给药区代谢物环丙沙星的消除半衰期较恩诺沙星长,平均为(1.67±0.20)h,平均滞留时间(MT)亦长。在本 试验条件下,建议临床休药期不少于3d。【结论】恩诺沙星吸收较迅速,代谢较快、清除较快,表明恩诺沙星 与环丙沙星在乳中不会形成残留。 关键词:恩诺沙星;代谢物;消除;奶牛;乳房炎 Depletion of Enrofloxacin and its Metabolite in the Milk of Lactating Cows LIAO Jie-dan'2, LI Shou-jun, DING Huan-zhong', LIU Wen-zil, YU Jing-xian, ZHANG Jia-hui, LIU Ya-hong (College of veterinary Medicine, South China Agricultural University, Guang=hou 510642, College of Life Science, Foshan University, Foshan 528000, Guangdong) Abstract: [Objective] Depletion of enrofloxacin and its metabolite in the milk were studied in mastitis cows after a single intramammary dose of 500 mg per quarter. Method] Concentration of enrofloxacin and its metabolite ciprofloxacin were determined by high-performance liquid chromatography with fluorescence detector and the concentration-time data were analyzed by statistical moment principle. (Result] Enrofloxacin and its metabolite ciprofloxacin were all at the peak after 2 hours in the administered quarter. The concentrations were(13 16+3. 10) ug ml and (2.79+0.94) ug ml, respectively. Enrofloxacin and its metabolite ciprofloxacin can not be detected after 12 hours and 24 hours. In the healthy quarter enrofloxacin was at the peak after 1 hour and the concentration was(0.19+0.02)ug- ml". Enrofloxacin can not be detected after 6 hours. Ciprofloxacin was at the peak fter 4 hours and the concentration was (0.51+0.07) ug ml. Ciprofloxacin can not be detected after 24 hours. In the administered quarter, ciprofloxacin showed longer tinB which is (1.67+0.20) h in milk and maintained longer period than enrofloxacin. Under the experiment condition, it is suggested that the clinical withdrawal time should not be less than three days. [Conclusion] The results showed that enrofloxacin in mastitis cows is of rapid absorption, metabolism and elimination. Enrofloxacin and its metabolite rofloxacin are not residuary in milk Key words: enrofloxacin; metabolite; depletion; cows; mastitis 0引言 奶牛乳中恩诺沙星( enrofloxacin,ENR)及其代谢产 物环丙沙星( ciprofloxacin,CP)的浓度,研究乳房 【研究意义】采用高效液相色谱荧光检测法测定炎奶牛给药后不同时间恩诺沙星及其代谢产物环丙沙 收稿日期:2008-01-16:接受日期:2008-12-08 基金项目:国家自然科学基金(30471308);广州市科技计划项目(2005J1C0111) 作者简介:廖洁丹(1979—),女,广东潮州人,硕士,研究方向为兽医药理学与毒理学。Tel:0757-85505213: E-mail com。通信 作者刘雅红(1966-),女,黑龙江泰来人,教授,博士,研究方向为兽医药理学与毒理学。Tel:020-85287189 o1994-2009chinaAcademicJournalElectronicpUblishingHouse.Allrightsreservedhttp://www.cnki.net中国农业科学 2009,42(1):312-317 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.01.040 收稿日期：2008-01-16；接受日期：2008-12-08 基金项目：国家自然科学基金（30471308）；广州市科技计划项目（2005J1-C0111） 作者简介：廖洁丹（1979－），女，广东潮州人，硕士，研究方向为兽医药理学与毒理学。Tel：0757-85505213；E-mail：liaojiedan@163.com。通信 作者刘雅红（1966－），女，黑龙江泰来人，教授，博士，研究方向为兽医药理学与毒理学。Tel：020-85287189；E-mail：gale@scau.edu.cn 恩诺沙星及其代谢产物在奶牛乳中的消除规律 廖洁丹 1,2，李守军 1 ，丁焕中 1 ，刘文字 1 ，余静贤 1 ，张嘉慧 1 ，刘雅红 1 （1 华南农业大学兽医学院，广州 510642；2 佛山科学技术学院，广东佛山 528000） 摘要：【目的】研究乳房炎奶牛单剂量乳房灌注 1%恩诺沙星（50 ml/头）后乳中药物浓度的变化规律。【方 法】采用 HPLC 法测定乳中恩诺沙星及其代谢产物环丙沙星的浓度，用统计矩原理处理药物浓度—时间数据。【结 果】在给药区恩诺沙星和代谢产物环丙沙星均于给药后 2ｈ左右乳中浓度达到最高值，分别为（13.16±3.10）µg·ml-1 和（2.79±0.94）µg·ml-1，然后逐渐下降；12 ｈ后检测不到恩诺沙星，24 ｈ已检测不到环丙沙星。对于非给药 区，恩诺沙星给药后 1ｈ左右乳中浓度达到最高值（0.19±0.02）µg·ml-1，然后逐渐下降；6 ｈ后检测不到恩诺沙 星，而代谢产物环丙沙星于给药后 4 ｈ乳药浓度可达最高峰（0.51±0.07）µg·ml-1，24 ｈ已检测不到乳药浓度。 给药区代谢物环丙沙星的消除半衰期较恩诺沙星长，平均为（1.67±0.20）h，平均滞留时间（MRT）亦长。在本 试验条件下，建议临床休药期不少于 3 d。【结论】恩诺沙星吸收较迅速 ,代谢较快、清除较快 ,表明恩诺沙星 与环丙沙星在乳中不会形成残留。 关键词：恩诺沙星；代谢物；消除；奶牛；乳房炎 Depletion of Enrofloxacin and Its Metabolite in the Milk of Lactating Cows LIAO Jie-dan1,2, LI Shou-jun1 , DING Huan-zhong1 , LIU Wen-zi1 , YU Jing-xian1 , ZHANG Jia-hui1 , LIU Ya-hong1 ( 1 College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642; 2 College of Life Science, Foshan University, Foshan 528000, Guangdong) Abstract: 【Objective】 Depletion of enrofloxacin and its metabolite in the milk were studied in mastitis cows after a single intramammary dose of 500 mg per quarter. 【Method】 Concentration of enrofloxacin and its metabolite ciprofloxacin were determined by high-performance liquid chromatography with fluorescence detector and the concentration-time data were analyzed by statistical moment principle. 【Result】 Enrofloxacin and its metabolite ciprofloxacin were all at the peak after 2 hours in the administered quarter. The concentrations were (13.16±3.10) µg·ml-1 and (2.79±0.94) µg·ml-1, respectively. Enrofloxacin and its metabolite ciprofloxacin can not be detected after 12 hours and 24 hours. In the healthy quarter enrofloxacin was at the peak after 1 hour and the concentration was (0.19±0.02) µg·ml-1. Enrofloxacin can not be detected after 6 hours. Ciprofloxacin was at the peak after 4 hours and the concentration was (0.51±0.07) µg·ml-1. Ciprofloxacin can not be detected after 24 hours. In the administered quarter, ciprofloxacin showed longer t1/2β which is (1.67±0.20) h in milk and maintained longer period than enrofloxacin. Under the experiment condition, it is suggested that the clinical withdrawal time should not be less than three days. 【Conclusion】 The results showed that enrofloxacin in mastitis cows is of rapid absorption, metabolism and elimination . Enrofloxacin and its metabolite ciprofloxacin are not residuary in milk. Key words: enrofloxacin; metabolite; depletion; cows; mastitis 0 引言 【研究意义】采用高效液相色谱荧光检测法测定 奶牛乳中恩诺沙星（enrofloxacin，ENR）及其代谢产 物环丙沙星（ciprofloxacin，CIP）的浓度，研究乳房 炎奶牛给药后不同时间恩诺沙星及其代谢产物环丙沙