四、呼吸链的电子传递在线粒体内膜两侧形成质 子梯度( A proton gradient across the inner membrane of mitochondria is generated using the electron motive force e An estimate of 10 protons are pumped for oxidizing one NADH and 6 for one FADh2 accompanying the electron flow through complexes I, III and iv e Conformational changes induced by electron transferring is believed to be coupled to proton pumping(however, the actual mechanisms is yet revealed e In actively respiring mitochondria, the measured Aph is about 0.75 and difference in electrical potential (Ap)is about 0.15-0.2V e The energy stored in such an H* gradient can be used to synthesize atP or to do other work An estimate of 10 protons are pumped for oxidizing one NADH and 6 for one FADH2 accompanying the electron flow through complexes I, III and IV.  Conformational changes induced by electron transferring is believed to be coupled to proton pumping (however, the actual mechanisms is yet revealed!).  In actively respiring mitochondria, the measured pH is about 0.75 and difference in electrical potential () is about 0.15-0.2 V.  The energy stored in such an H+ gradient can be used to synthesize ATP or to do other work. 四 、呼吸链的电子传递在线粒体内膜两侧形成质 子梯度（A proton gradient across the inner membrane of mitochondria is generated using the electron motive force ）