五、呼吸链顺序的实验证据( The order of the many electron carriers on the respiratory chain have been elucidated via various studies) o Measurement of the standard reduction potential(AE( Electrons tend to transfer from low E0 carriers to high E0 carriers (but may deviate from this in real cells) Oxidation kinetics studies: Full reduction followed by sudden O introduction, earlier oxidation, closer to the end of the respiratory chain; using rapid and sensitive spectrophotometric techniques to follow the oxidation of the cytochromes, which have different wavelength of maximal absorption Effects of various specific inhibitors: those before the blocked step should be reduced and those after be oxidized Isolation and characterization of each of the multiprotein complexes: specific electron donors and acceptors can be determined for portions of the chain Measurement of the standard reduction potential (E`0)): Electrons tend to transfer from low E`0 carriers to high E`0 carriers (but may deviate from this in real cells).  Oxidation kinetics studies: Full reduction followed by sudden O2 introduction; earlier oxidation, closer to the end of the respiratory chain; using rapid and sensitive spectrophotometric techniques to follow the oxidation of the cytochromes, which have different wavelength of maximal absorption.  Effects of various specific inhibitors: those before the blocked step should be reduced and those after be oxidized.  Isolation and characterization of each of the multiprotein complexes: specific electron donors and acceptors can be determined for portions of the chain. 五、呼吸链顺序的实验证据（The order of the many electron carriers on the respiratory chain have been elucidated via various studies）