11.5 Plasmids as Cloning Vectors Useful Properties of Plasmids as Cloning Vectors 1.Small size,easy to isolate and manipulate 2. Independent origin of replication,independently from direct chromosomal control?独立复制起点 3. Multiple copy number,making amplification of DNA possible:多拷贝数 4. Selectable markers,easy to detect and select the plasmid-containing clones:选择性标记 Chen Feng,Lecture of MicrobiologyChen Feng, Lecture of Microbiology 11.5 Plasmids as Cloning Vectors Useful Properties of Plasmids as Cloning Vectors 1. Small size, easy to isolate and manipulate 2. Independent origin of replication, independently from direct chromosomal control独立复制起点 3. Multiple copy number, making amplification of DNA possible多拷贝数 4. Selectable markers, easy to detect and select the plasmid-containing clones选择性标记