42 Chapter 3 For biosynthesis we can write: (1+o)CH Oy+=H O, 1 (Y-7(1+0)-)NADH+H) 23 →> CHa n+CO+H2O Where Y= the degree of reductance of biomass; Y the degree of reductance of nitrogen source Y= the degree of reductance of compound For oxidative phosphory lation we can write 2b(NADH +H)+bo -> 2b NAD+2bHO E-3.8 The coefficients a, b and c, which appear throughout these balance equations describe the extent to which these reactions occur relative to the growth reaction ie 1 +o)and re written taken into account elemental balances for each reaction How should the balance reactions already described for aerobic metabolism be adapted for anaerobic fermentations? 1)By deleting O everywhere 2) By dropping the oxidative phosphorylation reaction SAQ 3.2 Use the equations already given to predict how you might expect Yy to be 1)a decrease in the degree of reductance of substrate; 2)an increase in the efficiency of oxidative horvlation 3)a decrease in energy demand for biomass synthesis? Give reasons for your responses 3.2 Relationships between product formation and growth process When considering product formation stoichiometries it is essential to define the knetcs relationship between product formation and growth. Essentially, this classification divides microbial product production processes into four types42 Chapter 3 For biosynthesis we can write: (1 +dCH_I:Oy + -mOmNn n +? Cy,- *15(i+d---yN)(NADH+H+) 6 1 6 -> CH, 08 Na + a COZ + HzO E - 3.7 Where: y, = the degree of reductance of biomass; y, = the degree of reductance of nitrogen source; y = the degree of reductance of compound. For oxidative phosphorylation we can write: 2b (NADH + IT) + bQ -> 2b NAD' + 2bH20 E - 3.8 The coeffiaents u, b and c, which appear throughout these balance equations describe the extent to which these reactions occur relative to the growth reaction (ie 1 + a) and are written taken into account elemental balances for each reaction. n adapted for anaerobic fermentations? 1) By deleting OZ everywhere. 2) By dropping the oxidative phosphorylation reaction. How should the balance reactions already desaibed for aerobic metabolism be max Use the equations already given to predict how you might exped Y 'ys to be influenced by: 1) a decrease in the degree of reductance of substrate; 2) an increase in the efficiency of oxidative phosphorylation; 3) a decrease in energy demand for biomass synthesis? Give reasons for your responses. 3.2 Relationships between product formation and growth process khks When considering product fonnation stoichiometries it is essential to define the relationship between product formation and growth. Essentially, this classificatiun divides microbial product production processes into four types: