1. Digestion an Hydrolysis 2. PPT Protein with (NH4)2SO4 3. Centrifuge and Filter 4. Rupture of Pyridine Ring with CNBR (Cyanogen Bromide) 5. Color Formation with Sulfanilic Acid 6. Determine the Absorption at 470 nm 7. Plot the Standard Curve of Niacin vs. Concentration 8. (Straight Line of Best Fit) Niacin Determination Sample + Ca(OH) Autoclave 2 2 Hours1. Digestion an Hydrolysis 2. PPT Protein with (NH4)2SO4 3. Centrifuge and Filter 4. Rupture of Pyridine Ring with CNBR (Cyanogen Bromide) 5. Color Formation with Sulfanilic Acid 6. Determine the Absorption at 470 nm 7. Plot the Standard Curve of Niacin vs. Concentration 8. (Straight Line of Best Fit) Niacin Determination Sample + Ca(OH) Autoclave 2 2 Hours