SALMONELLA Med Brilliant Green Agar (CM 263 Oxoid) Ss Aga (CM 99 Oxoid) Selenite Broth (CM 395 Oxoid) Sodium biselenite LIZI OXoid) Media Preparation (a) Brilliant Green Agar: Dissolve 50gm of agar in 1 litre of distilled water by ringing to the boil, sterilize at 121 C for 15 minutes (b)SS Agar: Dissolve 63gm of agar in 1 litre of distilled water by bringing to the boil. DO NOT STERILIZE. Allow to cool to 50C and pour into sterile etri dishes (c) Selenite Broth: Dissolve 4gm of sodium biselenite in 1 litre of distilled water, add 19gm of selenite broth powder, dissolve, fill into containers to 5cm and sterilize for 10 minutes in a boiling water bath Method Prepare a 10% homogenate of 25gm of sample in 225ml of peptone water and incubate at 37'C for 24 hours Take 10mls of the homogenate after incubation and add to Selenite Broth incubate for 48 hours at 42"C. Streak plates containing Brilliant Green Agar and SS Agar after 24 and 48 hours ex Selenite Broth. Incubate plates at 37'C for 24 hours Salmonella will appear as pink colonies on BG agar and transparent colonies with black centres on SS agar.SALMONELLA Media Brilliant Green Agar (CM 263 Oxoid) SS Agar (CM 99 Oxoid) Selenite Broth (CM 395 Oxoid) Sodium Biselenite (LIZ1 Oxoid) Media Preparation (a) Brilliant Green Agar: Dissolve 50gm of agar in 1 litre of distilled water by bringing to the boil, sterilize at 121°C for 15 minutes. (b) SS Agar: Dissolve 63gm of agar in 1 litre of distilled water by bringing to the boil. DO NOT STERILIZE. Allow to cool to 50°C and pour into sterile petri dishes. (c) Selenite Broth: Dissolve 4gm of sodium biselenite in 1 litre of distilled water, add 19gm of selenite broth powder, dissolve, fill into containers to 5cm and sterilize for 10 minutes in a boiling water bath. Method Prepare a 10% homogenate of 25gm of sample in 225m1 of peptone water and incubate at 37°C for 24 hours. Take lOmls of the homogenate after incubation and add to Selenite Broth, incubate for 48 hours at 42°C. Streak plates containing Brilliant Green Agar and SS Agar after 24 and 48 hours ex Selenite Broth. Incubate plates at 37°C for 24 hours. Salmonella will appear as pink colonies on BG agar and transparent colonies with black centres on SS agar. 250