CLOSTRIDIUM PERFRINGENS Media Perfringens Agar (O P.S. P )-(CM543-Oxoid) A Supplement b (SR77-Oxoid) Media Preparation Dissolve 22.8gm of agar in 500mls of distilled water by bringing to the boil Sterilize for 15 minutes at 121 C, allow to cool to 50C and asceptically add the contents of one vial each of supplements a and b which have been rehydrated by the addition of 2ml distilled water Mix well and pour into sterile plates Method Pipette 1ml of the 10% homogenate onto a prepared plate and spread with a sterile spread Further dilutions may be used if the levels are high Incubate anaerobically for 18-24 hours at 37'C(a suitable gas jar and gas generating kits are available from Oxoid-HPO1lA, BRO38B, BRO42A and BRO55A). Count large black colonies and multiply x 10 to give a count per gnCLOSTRIDIUM PERFRINGENS Media Perfringens Agar (O.P.S.P.)-(CM543 - Oxoid) Supplement A (SR76 - Oxoid) Supplement B (SR77 - Oxoid) Media Preparation Dissolve 22.8gm of agar in 500mls of distilled water by bringing to the boil. Sterilize for 15 minutes at 121"C, allow to cool to 50°C and asceptically add the contents of one vial each of supplements A and B which have been rehydrated by the addition of 2ml distilled water. Mix well and pour into sterile plates. Method Pipette lml of the 10% homogenate onto a prepared plate and spread with a sterile spread. Further dilutions may be used if the levels are high. Incubate anaerobically for 18-24 hours at 37°C (a suitable gas jar and gas generating kits are available from Oxoid - HPOllA, BRO38B, BRO42A and BRO55A). Count large black colonies and multiply x 10 to give a count per gm. 25 I