3) capacitation(获能) and acrosome reaction(顶体反应) ot spermatozoa capacitation factor: secreted by epi, cells of oviduct, /capacity of releasing acrosome enzymes 4)quality and quantity of spermatozoa 2-6ml,100,000,000/ml, 1.5 ml; or <10.000.000: abnormal sperm >30% or capacity for mobile< 70% 5)meeting of sperm and ovum within 24 hrs3) capacitation （获能）and acrosome reaction（顶体反应） of spermatozoa • capacitation factor: secreted by epi. cells of oviduct, capacity of releasing acrosome enzymes 4) quality and quantity of spermatozoa: • 2-6 ml, 100,000,000/ml, • <1.5 ml; or <10,000,000; abnormal sperm >30%; or capacity for mobile< 70% 5) meeting of sperm and ovum within 24 hrs