Replication of DNA Addition of nucleotides to the growing chain Takes place in the 53'direction Catalyzed by DNA polymerase Key step is the addition of a nucleoside 5'-triphosphate to the free 3'-hydroxyl group of the growing chain,with loss of a diphosphate leaving group 000 5'end CH2OP-OPOPO 5'end 0-0 0 POCH2 O=POCH2 OH CH2OP=O CH2OP=O 5'end 5'end New strand ● 3'end 3'end Template strand Addition of nucleotides to the growing chain ▪ Takes place in the 5′→3′ direction ▪ Catalyzed by DNA polymerase ▪ Key step is the addition of a nucleoside 5′-triphosphate to the free 3′-hydroxyl group of the growing chain, with loss of a diphosphate leaving group Replication of DNA