水,而对照中加25 Oul fda。悬浮液涡旋振荡,取5m置于含5ml丙酮的试管中以终止反 应。9464×g离心5min,在490m波长下测定上清夜的光密度。FDA水解值通过以荧光素 浓度对光密度值制作的标准曲线查得(浓度范围0-10ug/ml) 参考文献 Taylor, J P, Wilson, B, Mills, M.S., Burns R.G., 2002. Comparison of microbial numbers and enzymatic activities in surface soils and subsoils using various techniques. Soil Biology and Biochemistry, 34, 387-401水，而对照中加 250ul FDA。 悬浮液涡旋振荡，取 5ml 置于含 5ml 丙酮的试管中以终止反 应。9464×g 离心 5min，在 490nm 波长下测定上清夜的光密度。FDA 水解值通过以荧光素 浓度对光密度值制作的标准曲线查得（浓度范围 0-10ug/ml） 参考文献 Taylor, J.P., Wilson, B., Mills, M.S., Burns, R.G., 2002. Comparison of microbial numbers and enzymatic activities in surface soils and subsoils using various techniques. Soil Biology and Biochemistry, 34, 387-401