EC001092674PHo46 BstAPI 179 Aatll 2617 Ndel 183 Sspl 2501 Ehel 235 Pdml 2294 Bcgl 2215 2486 146/ 236 MCS Scal 2177 lacZ 469. pUC18/19 BsaXI 659 Sapl 683 2686bp 852. Aflll,Pscl 806 Gsul 1784 1626 Cfr10l1779 1466 Ec03111766 Eam11051694, ep (pMB1) BseYl 1110 Cail 1217