核农学 15卷 1050 培养基■ediu 图2不同培养基及激素配比对愈伤组织再分化的影响 Fig. 2 Eifects on regeneration of callus in different medium with different rate of kinetin 奇数为B3培养基上愈伤组织再分化数偶数为P3培养基上愈伤组织的再分化数 The odd numbers showed that the regeneration number of callus cultured in Bs medium iseries 1) The even numbers showed that the regeneration number of callus cutured :n is medium:(ser.es 2) 图版IL. fender与B.maps原生质体融和 Plate I The protoplast fusion between L fendler and B. napus 大的原生质体来自B.ms;小的来自L. fender The big protoplast was the one of B. napus; The small protoplast was the one of L. fender 刚移入田间时生长略缓慢,成熟时株高比对照微矮。L. fendleri和N.nlps不论是原生质体在 融合时用γ射线处理或PEG处理,还是非处理对照,其杂种融合原生质体细胞再生植株移入 大田后,初植阶段生长缓慢,植株高度60~70cm,基杆粗细介于双亲之间,叶片形状类似L fendler,但叶片大小是其2倍以上,花型、花色与N.mps相同,花药大小则处于两亲本之间 略偏于N.ns,花药空瘪,无花粉粒,自交不能结实(图版Ⅲ。 201994-2008ChinaAcademicJOurnalElectronicPublishingHouseAllrightsreservedhtp/iwww.cnki.net图 2 不同培养基及激素配比对愈伤组织再分化的影响 Fig. 2 Effects on regeneration of callus in different medium with different rate of kinetin 奇数为 B5 培养基上愈伤组织再分化数 ;偶数为 P8 培养基上愈伤组织的再分化数。 The odd numbers showed that the regeneration number of callus cultured in B5 medium (series 1) ; The even numbers showed that the regeneration number of callus cultured in P8 medium (series 2) 图版 Ⅰ L . fendleri 与 B . napus 原生质体融和 Plate Ⅰ The protoplast fusion between L . fendleri and B . napus 大的原生质体来自 B . napus ; 小的来自 L . fendleri 。 The big protoplast was the one of B . napus ; The small protoplast was the one of L . fendleri 刚移入田间时生长略缓慢 ,成熟时株高比对照微矮。L . fendleri 和 N. nupus 不论是原生质体在 融合时用γ射线处理或 PEG处理 ,还是非处理对照 ,其杂种融合原生质体细胞再生植株移入 大田后 ,初植阶段生长缓慢 ,植株高度 60～70cm ,基杆粗细介于双亲之间 ,叶片形状类似 L . fendleri ,但叶片大小是其 2 倍以上 ,花型、花色与 N. nupus 相同 ,花药大小则处于两亲本之间 , 略偏于 N. nupus ,花药空瘪 ,无花粉粒 ,自交不能结实(图版 Ⅱ) 。 843 核 农 学 报 15 卷 © 1994-2008 China Academic Journal Electronic Publishing House. All rights reserved. http://www.cnki.net