Review of protein structure hierarchy e Primary structure Glycine(Gly, G) Alanine (Ala, A) Valine(VaL v Leucine(Leu, L) Isoleucine (e, I) MAAAAAAGPEMVRGQVE 20 amino acids Phenylalanine (Phe, F Proline (Pro, Pl Methionine (Met, M) Tryptophan (Trp, w) hydrophobic/hydrophilic Polar, Hydrophillic R-groups acidic/basic large/small specialized(Gly, Pro, Cys) Serine(Ser, S) Threonine(Thr, T) Cysteine(Cys, C) Tyrosine (, Y) Asparagine (Asn, N) amine( GIn, O Electrically char ENIL. Lysine( Lys, K) Aspartic acid (Asp, D) Arginine (Arg, RReview of protein structure hierarchy • Primary structure MAAAAAAGPEMVRGQVF • 20 amino acids – hydrophobic/hydrophilic – acidic/basic – large/small – specialized (Gly,Pro,Cys) C C O- O H Glycine (Gly, G) Alanine (Ala, A) Valine (Val, V) H H3 N+ C C O- O H CH3 CH3 CH3 H3 N+ C C O- O H CH H3N+ C C O- O H CH H3N+ H3C OH CH3 CH3 CH3 C C O- O H CH2 CH2 CH3 C C O- O H H3N+ CH2 CH2 C C O- O H H3 N+ CH2 S CH3 H3N+ C C O- O H H3 N+ CH2 C C O- O O H H3 N+ CH2 C C O- O H H3 N+ CH2 CH2 C NH2 C C C O- O H H3 N+ CH2 C C O- O H H2 N+ H2C C C O- O O H H3N+ CH2 C C O- O H H3 N+ CH2 CH2 - O C O C C O- O H H3 N+ CH2 CH2 CH2 CH2 NH3 + NH2 + NH+ NH C C O- O H H3N+ CH2 C C O- O H H3 N+ CH2 CH2 CH2 C NH NH2 - O C NH2 O CH2 CH NH 2 C C O- O H H3 N+ CH2 C C O- O H H3N+ CH2 SH OH C C O- O H H3 N+ CH OH Leucine (Leu, L) Isoleucine (IIe, I) Methionine (Met, M) Serine (Ser, S) Aspartic acid (Asp, D) Glutamic acid (Glu, E) Lysine (Lys, K) Arginine (Arg, R) Histidine (His, H) Threonine (Thr, T) Cysteine (Cys, C) Tyrosine (Tyr, Y) Asparagine (Asn, N) Glutamine (Gln, Q) Acidic Basic Phenylalanine (Phe, F) Tryptophan (Trp, W) Proline (Pro, P) Electrically charged Polar, Hydrophillic R-groups Nonpolar, Hydrophobic R-groups