Stephen M. Mount Cell Biology and Molecular Genetics H. J. Patterson Hall University of Maryland College Park, MD 20742-5815 Phone301-405-6934 FAX301-314-9081 permanent email addresssm 193@umail umd. edu This is Steve Mount's web page for gene annotation and splice site selection. Much of the material here is relevant to a review article in the american journal of Human Genetics Annotation Gene annotation incorporates cdna data(including ESTS); sequence similarity; and computational predictions based on the recognition of probable splice sites and coding regions. The state of the art was recently surveyed by the Gene Annotation Assessment Project(GASP1), the results of which were published in a special issue of Genome Research Ensembl- Ensembl is a joint project between EMBL-EBI and the Sanger Centre to develop a software system which produces and maintains automatic annotation on eukaryotic genomes Gene Ontology Consortium-The goal of the Gene Ontology Consortium is to produce a dynamic controlled vocabulary that can be applied to all eukaryotes even as knowledge of gene and protein roles in cells is accumulating and changing Oak Ridge National Laboratory Computational Biosciences Section-A project whose stated mission is to address fundamental questions in the life sciences and provide infomation and analytical resources to the wider biology research community. TIGR Databases The Institute for mic Research tigr databases are a collection of curated databases containing DNA and protein sequence, gene expression, cellular role, protein family, and taxonomic data for micr ants and humans Celera- Celera genomics, a division of PE Corporation, which produced the fruit fly and human genomes. They are currently working on the mouse, and have announced a plan to move into proteomIcs GenefindingStephen M. Mount Cell Biology and Molecular Genetics H. J. Patterson Hall University of Maryland College Park, MD 20742-5815 Phone301-405-6934 FAX301-314-9081 permanent email addresssm193@umail.umd.edu This is Steve Mount's web page for gene annotation and splice site selection. Much of the material here is relevant to a review article in the American Journal of Human Genetics. Annotation Gene annotation incorporates cDNA data (including ESTs); sequence similarity; and computational predictions based on the recognition of probable splice sites and coding regions. The state of the art was recently surveyed by the Gene Annotation Assessment Project (GASP1), the results of which were published in a special issue of Genome Research. Ensembl - Ensembl is a joint project between EMBL-EBI and the Sanger Centre to develop a software system which produces and maintains automatic annotation on eukaryotic genomes. Gene Ontology Consortium - The goal of the Gene Ontology Consortium is to produce a dynamic controlled vocabulary that can be applied to all eukaryotes even as knowledge of gene and protein roles in cells is accumulating and changing. Oak Ridge National Laboratory Computational Biosciences Section - A project whose stated mission is to address fundamental questions in the life sciences and provide information and analytical resources to the wider biology research community. TIGR Databases - The Institute for Genomic Research TIGR Databases are a collection of curated databases containing DNA and protein sequence, gene expression, cellular role, protein family, and taxonomic data for microbes, plants and humans. Celera - Celera genomics, a division of PE Corporation, which produced the fruit fly and human genomes. They are currently working on the mouse, and have announced a plan to move into proteomics. Genefinding