1.4 Target Molecular Dynamics (TMD) Periplasmic side Periplasmic side C MI SI-MI a123 Leul9 N Cytoplasmic side S1-MI linker Phe10 S Phe 7 N Cytoplasmic side Fig.2.Schematic illustration of the tandem layers of hydrophobic lining of the gate that form the hydrophobic constrictions of the inner pore of the channel.For clarity,only two subunits are shown,and the M1 helices are drawn in parallel although they are packed at an angle in real structure.All other structural elements are omitted. Closed Open Fg.1.Overall architecture of modeled EcoMscL.Both the closed (Left)and open(Right)forms are showninside vlew(Upper)andtopvlew (Lower).Majo secondary structural elements are color-coded:yellow for M1,green for M2, blue for s1,red for 52,and orange for S3.The figures are made by graphic software MOLSCRIPT (28)and rendered by RASTE3D (29). Moy-bU八I Yifei Kong,Yufeng Shen,Tiffany E.Warth and Jianpeng Ma(2002).Conformational Pathways in the Gating of E.colt Mechanosensitive Channel.Proc.Natl.Acad.Sci.USA.99:5999-6004 1.4 Target Molecular Dynamics（TMD） Yifei Kong, Yufeng Shen, Tiffany E. Warth and Jianpeng Ma (2002). Conformational Pathways in the Gating of E.coli Mechanosensitive Channel. Proc. Natl. Acad. Sci. USA. 99: 5999-6004