Biocatalysts in organic chemical synthesis Esterase enzymes- cleavage of various ester bonds to yield an acid and an alcohol, ie RI COoR2+H20 <- →>R1COOH+R2OH LIpase enzymes(a subgroup of esterase enzymes)-hydrolyse fats into glycerol and fatty acids, eg H2C-, H,COH R1 OOH 2+929 HCOH R2OOH H2C- OR H2CO fatty acids Proteolytic enzymes-hydrolyse proteins selectively, either on terminal groups (exopeptidases)or intemal linkages(endopeptidases),eg A Oxldoreductases-catalyse oxidation-reduction reactions, eg dehydrogenase H.C. CH,OH →H2C.CHo NAD+ NADH +H+ Oxygenases- add one(monooxygenases)or both(dioxygenases)atoms of molecular oxygen to molecules, eg NADH NAD+ cis-dihydrodiol Table 2.1 Some reactions catalysed by microbial enzymes. In principle each enzyme catalyses the reverse process as wellBiocatalysts in organic chemical synthesis 11 Table 2.1 Some reactions catalysed by microbial enzymes. In principle each enzyme catalyses the reverse process as well