2、分辨力和光镜的分辨极限 分辨力( Resolution):又叫分辨本 领,是指将邻近两点清晰区分辨认的 能力。 microscop cone of ill n and therefore on both he cond the objective lens. It is he formula s a co where 0= half the angular width of the cone of ondenser lens from a typical point in the specimen ses a con since the maximum width is 180 rays each point of the ns the refractive index of the medium specimen usually air or oil) separating the LIGHT specimen from the objective and ondenser lenses 2. the wavelength of light used (for white light, a figure of 0.53 um is commonly assumed) NUMERICAL APERTURE: n sin e in the aperture, the greater the resolution and the quation above is called the numerical aperture brighter the image (brightness is important in of the lens (NA) and is a function of its fluorescence microscopy ). However this advan collecting ability For dry lenses this cannot be tago is obtained at the exponse of vory shor more than 1, but for oil-immersion lenses it can working distances and a very small depth of field be as high as 1. 4. The higher the numerical2、分辨力和光镜的分辨极限 分辨力（Resolution）：又叫分辨本 领，是指将邻近两点清晰区分辨认的 能力