Contents 1 Fermentation Pilot Plant Yujiro Harada, Kuniaki Sakata, Seiji Sato and Shinsaku Takayama PROLOGUE(by Yujiro Harada) 1.0 MICROBIAL FERMENTATION (by Kuniaki Sakato 1.1 Fermentation Pilot Plant 1.2 Bioreactors and Culture Techniques 233 for microbial Processes 1. 3 Application of Computer Control and Sensing Technologies for Fermentation Process 1.5 Bioreactors for Recombinant DNA Technology.... 22 2.1 Introduction 2.2 Culture media 2.3 Microcarrier Culture and General Control Parameters. 26 2.4 Perfusion Culture Systems as a New High Density Culture Technolog 2.5 Sedimentation Column Perfusion Systems 2.6 High Density Culture Using a Perfusion Culture System with Sedimentation Column 2.7 Acknowledgment References and Bibliography (Section 2)Contents 1 Fermentation Pilot Plant ........................................ 1 Yujiro Harada, Kuniaki Sakata, Seiji Sat0 and Shinsaku Takayama PROLOGUE (by Yujiro Harada) ................................................. 1 1 .O MICROBLAL FERMENTATION (by Kuniaki Sakato) ........ 2 ........... 3 ........... 3 1.1 Fermentation Pilot Plant ...... 1.2 Bioreactors and Culture Techniques 1.3 Application of Computer Control and 1.4 Scale-up ................................................... 1.5 Bioreactors for Recombinant DNA Technolo References (Section 1) ....................................... 2.0 MAMMALIAN CELL CULTURE SYSTEM (by 2.1 Introduction ............................... ......................... 25 2.2 Culture Media ........................................ 2.3 Microcarrier Culture and General Contro 2.4 Perfision Culture Systems as a New High Density for Microbial Processes ........................ Sensing Technologies for Fermentation Process ............... 8 Culture Technology ....................................................... 3 1 2.5 Sedimentation Column Perfision Systems ...................... 33 2.6 High Density Culture Using a Perfision Culture System with Sedimentation Column. 2.7 Acknowledgment ................. References and Bibliography (Se xiii