8/24/2016 Sample Collection and Processing Sample Collection and Processing Sample collection should not introduce microbes A sample will only yield significant and into the food sample meaningful results if Best,if individual containers are submitted for 1.It represents the mass of the analysis material sampled If not,use aseptic technique to collect a sample 2.The sample is handled in a manner Instruments used to sample should be sterile that prevents changes in the Containers(bags or wide-mouthed jars)should microbial numbers between collection be sterile and analysis Containers should be labeled with food name, date collected,and other information used to track or analyze the results Sample Collection and Processing Sample Preparation ·Sample should be Samples should be analyzed as quickly as possible to prevent any change in the microbial population of ba If this is not possible: -Samples should be refrigerated ender for large volume food Frozen samples should be kept frozen testing procedures -Products normally refrigerated should such as FDA Bacteriological never be frozen as this may cause Analytical Manual (BAM) microbial death 38/24/2016 3 Sample Collection and Processing A sample will only yield significant and meaningful results if 1. It represents the mass of the material sampled 2. The sample is handled in a manner that prevents changes in the microbial numbers between collection and analysis 9 Sample Collection and Processing Sample collection should not introduce microbes into the food sample • Best, if individual containers are submitted for analysis • If not, use aseptic technique to collect a sample • Instruments used to sample should be sterile • Containers (bags or wide-mouthed jars) should be sterile • Containers should be labeled with food name, date collected, and other information used to track or analyze the results 10 Sample Collection and Processing Samples should be analyzed as quickly as possible to prevent any change in the microbial population If this is not possible: - Samples should be refrigerated - Frozen samples should be kept frozen - Products normally refrigerated should never be frozen as this may cause microbial death 11 Sample Preparation • Sample should be processed to produce a homogeneous suspension of bacteria that can be pipetted • Stomacher - a laboratory blender for large volume food testing procedures, such as FDA Bacteriological Analytical Manual (BAM) 12