Flash column chromatography is a quick and (usually) easy way to separate complex mixtures of compounds. We will be performing relatively large scale separations in 5.301,around 1.0 g of compound. Columns are often smaller in scale than this and some of you will experience these once you move into the research lab. Column chromatography uses the same principles

This handout describes how to prepare a standard gas chromatograph sample. It involves preparing a dilute solution of a somewhat volatile compound and using the GC to assess its purity

Working on a vacuum manifold: See LLP chapter 9.2 for an excellent discussion on working with air-sensitive reagents, including how to use a two-way manifold. De-gassing solvents: The best way to remove water and oxygen from a solvent is to distill it over an appropriate drying agent (such as sodium). This can sometimes be a lengthy (and dangerous)task. Since we

For a two-solvent recrystallization, you should have one solvent (solvent #1) in which your desired compound is soluble at the boiling point. The second solvent(solvent #)should induce crystallization when added to a saturated solution of your compound in the primary solvent. Reference:

X-Ray diffraction is an important and powerful tool for determining the solid state structure of compounds. Modern advances have made data collection and structure solution almost routine for many small molecules To use this technique, however, good quality single crystals are still needed. In this exercise you will experiment with the art of growing single crystals

This is two-day- experiment. Do not start this experiment unless you have two free lab days to complete it.*** Techniques Checklist: Picking the correct eluent Adsorbtion of a crude mixture onto silica gel Separating complex mixtures using gradient elution Pre-lab Discussion: Suggest limited list of eluent solvent systems Discuss sample adsorption and gradient elution strategies

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1. volumetric Techniques: Don' t let inaccuracy hold you back in lab! This video introduces the proper methods for measuring precise volumes of liquid using pipets, burets, volumetric flasks, and syringes

Techniques Checklist m manifold use Manipulations under an inert atmosphere Solvent de-gassing Pre-Lab Discussion and required reading: Air sensitive techniques