1. Please describe the main features of the DNA structure. (10 points) Key features: (1) Double helical structure (4’) (2) Two strands are held together in an antiparallel orientation (3’) by base paring (1’)

Describing the following terms (4 points each) 1. Cre recombinase 2. CSSR recombination 3. Mating-type switching 4. Mismatch repair 5. Polymerase switching 6. 30-nm fiber 7. Core histones

Recombinant gene expression in eukaryotic systems is often the only viable route to the large-scale production of authentic, post￾translationally modified proteins. It is becoming increasingly easy to find a suitable system to overexpress virtually any gene product

How Important Is Cost? Reagents obviously figure into the cost of a procedure, but the labor required to produce and apply the reagents of purification should also be considered. How Important Is Reproducibility (Robustness) of the Procedure?

一、三种不同的RNA聚合酶 二、RNA 聚合酶的组成 三、真核RNA聚合酶的活性

一、转录反应和转录方向 二、摸板链（-）和编码链（+）p.166 三、转录步骤：起始,延伸,终止

Translation is the conversion of the information in the nucleic acid sequence to polypeptides of a specific amino acid sequence

Lecture 7: Hydrogel Biomaterials: Structure and Physical Chemistry Last Day: programmed/regulated/multifactor controlled release for drug delivery and tissue engineering

Lecture 6: Biodegradable Polymers for Tissue Engineering Last time: enzymatic degradation of solid polymers Engineering biological recognition of polymers Toda Designing polymers for tissue engineering Reading

Lecture 10: Bioengineering applications of hydrogels: Molecular Imprinting and Drug Delivery Last Day polyelectrolyte gels Polyelectrolyte complexes and multilayers Applications in bioengineering Theory of ionic gel swelling Toda Molecular imprinting