1. Enzymes Are Powerful and Highly Specific Catalysts 2. Free Energy Is a Useful Thermodynamic Function for Understanding Enzymes －热力学

1.The Exploration of Genes Relies on Key Tools 2.Recombinant DNA Technology Has Revolutionized All Aspects of Biology 3.Complete Genomes Have Been Sequenced and Analyzed 4.Eukaryotic Genes Can Be Manipulated with Considerable Precision

1.The Purification of Proteins Is an Essential First Step in Understanding Their Function 2. The Characterization of Proteins: Molecular Weight, isoelectric point. 3 . Amino Acid Sequencing and primary structure

1. Biochemical Unity Underlies Biological Diversity 2. DNA Illustrates the Interplay between Form and Function 3. Concepts from Chemistry Explain the Properties of Biological Molecules 4.The Genomic Revolution Is Transforming Biochemistry and Medicine

D1 The immune system D2 Antibody structure D3 Polyclonal and monoclonal antidies D4 Antibody synthesis D5 Antibodies as tools

1. DNA structure 2. Chromosomes 3. DNA replication in bacteria 4. DNA replication in eukaryotes

E1.Membrane lipids E2.Membrane protein and carbohydrate E3.Membrane transport:small molecules E4.Membrane transport:macromolecules E5.Signal transduction

C1 Introduction to enzymes C2 Thermodynamics热力学 C3 Enzyme kinetics动力学 C4 Enzyme inhibition C5 Regulation of enzymeactivity

A1 Prokaryotes A2 Eukaryotes A3 Microscopy A4 Cellular fractionation

第一节代谢调节的概念 第二节物质代谢的相互联系 第三节代谢调节